目的 Dof(DNA binding with one finger)基因家族是植物特有转录因子基因家族之一,在植物生长发育和其他非生物胁迫响应中起重要调控作用。本研究以工业大麻基因组中的Dof转录因子作为研究对象,针对Dof基因家族的基因结构、染色体定位、编码蛋白的理化性质及系统发育等方面进行分析。方法 本研究通过生物信息学手段对工业大麻Dof基因进行鉴定和分析,使用PlantTFDB、ExPASy、MEME、WoLFPSORT、PlantCare等在线网站及TBTools、MEGA、Clustal、Gendoc等软件进行预测、可视化分析。结果 研究发现,大麻中含有27个Dof基因成员,命名为CsDof1~CsDof27;染色体定位分析结果表明,27个Dof基因不均匀地分布在9条染色体上。由理化性质分析可知,氨基酸数目在185~512 aa之间,等电点介于4.91~9.22之间,所有Dof基因定位在细胞核中;通过对大麻和拟南芥Dof蛋白的系统发育分析,Dof基因家族可以被分为9个亚组。结论 大麻Dof基因具有多个顺式作用元件,转录组数据表达热图显示部分大麻Dof基因具有茎和花组织表达特异性,未来研究将重点放在大麻花序中特异性表达的Dof基因上的功能研究。
Abstract
OBJECTIVE To analyze the Dof transcription factor gene family of Cannabis sativa L. in aspects of gene structure,chromosome localization, physicochemical properties and phylogeny of coding proteins. METHODS In this study, the Dof gene of C. sativa was identified and analyzed by means of bioinformatics. Online websites such as PlantTFDB, ExPASy, MEME, WoLFPSORT, PlantCare and software such as TBTools, MEGA, Clustal and Gendoc were used for prediction and visual analysis. RESULTS The results showed that there were 27 Dof gene members in C. sativa named CsDof1~CsDof27. The results of chromosome localization analysis showed that 27 Dof genes were unevenly distributed in 9 linkage groups. According to the analysis of physical and chemical properties, the number of amino acids ranged from 185 to 512aa, and the isoelectric points ranged from 4.91 to 9.22. Through phylogenetic analysis of Dof proteins in C. sativa and A. thaliana, the Dof gene family can be divided into 9 subgroups. CONCLUSION The CsDof gene has multiple cis-action elements. Transcriptome expression heat map shows that some CsDof genes have specific expression in stem and flower tissues.Future research will focus on the functional study of the specifically expressed Dof genes in the inflorescence of C. sativa.
关键词
大麻 /
Dof基因 /
转录因子 /
进化分析
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Key words
Cannabis sativa L /
Dof gene /
transcription factors /
evolutionary analysis
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